BiOligo
Super-quenched qPCR Probes
Super-quenched qPCR Probes
To reduce background fluorescence caused by incomplete quenching in traditional TaqMan probes, we developed the Super-Quenched (SQ) probe. By adding an extra internal quencher and a unique 3' quencher, this design effectively lowers background noise, improves sensitivity, and prevents fluorescence leakage.
Features
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High Sensitivity | LOD to 1 copy
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High Signal-to-Noise Ratio (SNR) | Effectively reduces background fluorescence, increase endpoint fluorescence signal intensity
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Ideal for Multiple qPCR Detection | Minimizes fluorescence crosstalk, minimize non-specific amplification
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Applicable to AT-rich Targets
Recommended Combinations
Cases
Comparison of Modified Fluorescence for Different Quenchers in the Same Sequence.
qPCR Applicable —— Lower background signal and higher SNR
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Channel
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Target
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FAM
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AsFV-p72
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HEX
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Monkeypox plasmid
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Cy5
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COVID-19
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