Anti-DNP mouse IgG1 isotype control antibody

Background

Because Dinitrophenyl (DNP) is a hapten and not found endogenously in animal and plant tissues, the anti-DNP monoclonal antibody in nature is suitable for use as an isotype-matched control antibody to estimate the degree of non-specific binding by an antigen-specific antibody. The recombinant mouse IgG1 kappa isotype control (clone number: DNP.m1) and mutants (natural and engineered mIgG1 isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study. The isotypes of mouse IgG antibody reagents used in scientific research and clinical diagnostics are mainly mouse IgG1 kappa. Mouse IgG1 antibodies and engineered mutants are also used in therapeutic drug discovery. Mouse IgG1 antibodies are more like human IgG4 antibodies than human IgG1 antibodies, which behave more like mouse IgG2a or IgG2c antibodies. Many antibody drugs especially those targetting immune checkpoint proteins use the human IgG4 constant regions.

As an ideal recombinant mouse IgG1 isotype control,  the recombinant anti-DNP mIgG1 kappa isotype control has low or no specific binding to any mouse sample, and has completely mouse variable regions and the mouse IgG1 kappa constant regions. The anti-DNP mouse IgG1 kappa isotype control and mutants can be used in ELISA, WB, IHC, the cell-based assay or animal model as the antibody negative control.

Please contact us to ask for a quote for the engineered anti-DNP isotype control mutants, isotype control and mutants with Avi-, His-, and Flag-tags, and biotinylated isotype control and mutants. A variety of conjugates (such as dyes and fluorophores) with the anti-DNP isotype control and mutants are available.

In Vivo Grade Recombinant Anti-DNP Mouse IgG1 Kappa Isotype Control Antibody (mIgG1 Isotype Control)

The in vivo grade recombinant anti-DNP mouse IgG1 isotype control antibody (mIgG1 isotype control) was produced in mammalian cells.
Specificity: Dinitrophenyl (DNP).
Clone: DNP.m1.
Isotype: mouse IgG1, kappa.
Applications: an isotype-matched negative control for anti-DNP mouse IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The in vivo grade recombinant anti-DNP mouse IgG1 kappa isotype control antibody (clone of DNP.m1) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.