Anti-HEL VHH-hIgG1 Fc isotype control antibody

Background

The anti-HEL VHH (hen egg-white lysozyme; nanobody, single-domain antibody, or the variable domain of heavy chain antibody) in nature is suitable for use as an isotype-matched control antibody to estimate the degree of non-specific binding by an antigen-specific antibody. As an ideal recombinant VHH isotype control antibody,  the recombinant anti-HEL VHH isotype control antibody has low or no specific binding to any human and mouse sample. The recombinant anti-HEL VHH isotype control antibodies are useful for in vitro and in vivo studies from ELISA, cell-based assay to animal model study.

Please contact us to ask for a quote for the engineered anti-HEL VHH isotype control antibodies with His- and Fc-tags. A variety of conjugates (such as dyes and fluorophores) with the anti-HEL isotype control and mutants are available.

 In Vivo Grade Recombinant Anti-HEL VHH-His Isotype Control Antibody

The in vivo grade recombinant anti-HEL VHH isotype control antibody with the His-tag was produced in mammalian cells.
Specificity: hen egg-white lysozyme (HEL).
Clone: D3-L11.
Isotype: VHH.
Applications: an isotype-matched negative control for anti-HEL VHH antibody used in ELISA, Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007165.h1: In Vivo Grade Recombinant Anti-HEL VHH-hIgG1 Fc Isotype Control Antibody

The in vivo grade recombinant anti-HEL VHH isotype control antibody with the hIgG1 Fc-tag was produced in mammalian cells.
Specificity hen egg-white lysozyme (HEL).
Clone: D3-L11.
Isotype: VHH.
Applications: an isotype-matched negative control for anti-HEL VHH antibody used in ELISA, Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The in vivo grade recombinant anti-HEL VHH isotype control antibodies (clone of D3-L11) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.