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    Yeasen

    BspQI GMP-grade (10 U/μL)

    BspQI GMP-grade (10 U/μL)

    $105.00
    $105.00
    Sale Sold out
    Size

    Description

    This product is a type II restriction endonuclease derived from the recombinant protein encoded by the BspQI gene in Bacillus sphaericus expressed by E.coli. Its recognition sequence is 5'-GCTCTTCN1/N4-3'. Use to digest plasmids to prepare poly (A/T/G/C)-terminated linearized DNA fragments to obtain specific cohesive ends. This product is produced in accordance with GMP process requirements and provided in a liquid form.

    Feature

    • Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
    • Restriction Enzyme Cut Site: GCTCTTC (1/4)
    • Digestion of DNA to obtain specific sticky ends
    • Isolated from a recombinant source
    • Tested for the absence of endonucleases, exonucleases, RNases

    Application

    • Digest the plasmid to prepare a linearized DNA fragment at the end of Poly (A/T/C/G);
    • Linearize plasmid template before in vitro transcription
    • Restriction digest

    Specification

    Expression Host Recombinant E. coli with BspQI gene
    Reaction Temperature 50℃
    Storage Buffer 20 mM Tris-HCl, 0.5 M KCl, 0.1 mM EDTA, 1mM DTT, 0.1% Triton X-100, 50% Glycerol
    Unit Definition 1 unit: The amount of enzyme required to digest 1 μg of λDNA within 1 h at 50℃ in a 50 μL system

    Components

    Components No. Name 10664ES76 (500 U) 10664ES86 (2,500 U) 10664ES92 (10 KU) 10664ES98 (100 KU)
    10664 BspQI GMP-grade (10 U/μL) 50 μL 250 μL 1 mL 10 mL

    Shipping and Storage

    The product is shipped with blue ice and can be stored at -15℃ ~ -25℃ for one year.

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