Apenzy Biosciences
CleanRNA™ eGFP-mRNA (low dsRNA)
CleanRNA™ eGFP-mRNA (low dsRNA)
Product description
eGFP is frequently utilized as a fluorescent tag for the observation and monitoring of protein expression and location within living cells. When eGFP-mRNA is transfected into cells, the enhanced green fluorescent protein (eGFP) is produced within the cellular environment. CleanRNA™ eGFP-mRNA (low dsRNA) is an mRNA that has been modified with a 5' cap1, a 3' poly(A) tail, and N1-Methyl-Pseudouridine Triphosphate (N1-Me-Pseudo UTP). The product is created through an in vitro transcription process using CleaScrip™ T7 RNA polymerase, which generates ultralow sRNA content in the mRNA solution, thus improving the in vivo performance of eGFP-mRNA.
Specifications
Catalog Number |
N120012S/N120012M |
Specifications |
0.5 mg/1 mg
|
mRNA length |
995 nt |
Concentration |
1 mg/mL |
Capping efficiency |
100% |
dsRNA |
<0.1 ng/μg |
Storage buffer |
1 mM sodium citrate |
Components
Component Number |
Component Name |
Storage Temperature |
N120011S |
N120011M |
N120012 |
CleanRNA™ eGFP-mRNA (low dsRNA) |
-80°C |
0.5 mg |
1 mg |
Integrity tested by CE (capillary electrophoresis)
Shipping and Storage
The products are shipped with dry ice and can be stored at -80℃ for two year.
Notes
1. Upon arrival, immediately store the samples at -80°C and avoid repeated freeze-thaw.
2. For the first use, be sure to wear disposable gloves, dissolve the samples on ice, and use RNase-free reagents and consumables to avoid contamination.
3. After the initial dissolution of the samples, they can be gently centrifuged and then aliquoted, avoiding repeated freezing and thawing.