Co-capping T7 in vitro transcription reagent (N1-Me-pUTP, CAP GAG)

Product Overview

The Co-Transcriptional Capping T7 In Vitro Transcription Kit is optimized for efficient transcription using T7 RNA polymerase. This kit enables in vitro transcription from linear double-stranded DNA templates containing a T7 promoter sequence, using NTPs as substrates. The capping reagent CAP GAG (3'OH) is co-transcriptionally incorporated into the 5' end of mRNA, allowing for the simple and rapid production of large quantities of RNA with a Cap1 structure.

The Cap1 structure protects mRNA from degradation, ensuring high translation efficiency. The kit also includes the modified nucleotide N1-Me-pUTP for transcription flexibility. These modifications effectively reduce mRNA immunogenicity and suppress innate immune activation, making the mRNA a potential tool for regenerative medicine, disease treatment, and cell reprogramming.

Each reaction can yield 150–200 μg of RNA and is scalable for milligram-level RNA production. The transcribed RNA is suitable for various applications, including RNA structure and function studies, RNase protection assays, probe hybridization, anti-sense RNA, and RNAi.

Transportation and Storage

• Transportation Method: Shipped at ≤0°C.
• Storage Conditions: Store at -25°C to -15°C.