Co-capping T7 in vitro transcription reagent (pUTP, CAP GAG)

Product Overview

The Co-Transcriptional Capping T7 In Vitro Transcription Kit is optimized for efficient transcription using T7 RNA polymerase. This kit enables in vitro transcription from linear double-stranded DNA templates containing a T7 promoter sequence, with NTPs as substrates. The capping reagent CAP GAG (3'OH) is co-transcriptionally incorporated into the 5' end of mRNA, enabling the simple and rapid production of large quantities of RNA with a Cap1 structure.

The addition of the Cap1 structure protects mRNA from degradation and ensures high translation efficiency. The kit includes commonly used modified nucleotides such as pUTP to meet specific transcription needs. These modifications effectively reduce mRNA immunogenicity and suppress innate immune activation, making mRNA a promising tool for regenerative medicine, disease treatment, and cell reprogramming.

Each reaction can yield 150–200 μg of RNA, and the system can be scaled up for milligram-level RNA production. The transcribed RNA is suitable for a variety of applications, including RNA structure and function studies, RNase protection assays, probe hybridization, anti-sense RNA, and RNAi.

Transportation and Storage

• Transportation Method: Shipped at ≤0°C.
• Storage Conditions: Store at -25°C to -15°C.