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Recombinant Deoxyribonuclease I (DNase I, RNase-free) 2 U/ μL
Recombinant Deoxyribonuclease I (DNase I, RNase-free) 2 U/ μL
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Description
DNase I is an endonuclease that can digest single- or double-stranded DNA. It can hydrolyze phosphodiester bonds to produce mono- and oligodeoxynucleotides containing a 5'-phosphate group and a 3'-OH group.
The optimal working pH range of DNase I is 7-8. The activity of DNase I depends on Ca2+ and can be activated by divalent metal ions such as Co2+, Mn2+, Zn2+, etc. In the presence of Mg2+, DNase I can randomly cleave any site of double-stranded DNA; while in the presence of Mn2+, DNase I can cleave DNA double-stranded at the same site, forming blunt ends or sticky ends with 1-2 nucleotides protruding.
This enzyme is derived from recombinant E. coli strains, does not contain RNase, and can be used for the treatment of various RNA samples.
Features
- Recombinant enzyme
- Stringent quality control
Applications
- Preparation of DNA-free RNA
- Removal of template DNA following in vitro transcription
- Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR
- DNA labeling by nick-translation in conjunction with DNA Polymerase I
- Studies of DNA-protein interactions by DNase I, RNase-free footprinting
Specifications
| Expression Host | Recombinant E. coli with Dnase I gene |
| Purity | ≥99% (SDS-PAGE) |
| Storage Buffer | 10 mM Tris-HCl (pH 7.6), 2 mM CaCl2, 50% glycerol |
| Unit Definition | The amount of enzyme required to completely degrade 1 μg of plasmid DNA at 37℃ for 10 mins |
Components
| Components No. | Name | 10325ES80 (1,000U) | 10325ES90 (5,000 U) |
| 10325-A | Recombinant DNase I (RNase-free) -2 U/μL | 500 μL | 5×500 μL |
| 10325-B | DNase I Reaction Buffer (10×) | 1 mL | 5×1 mL |
Storage
The product should be stored at -25℃ ~ -15℃ for two years. Please avoid repeated freeze-thaw.
Documents:
Safety Data Sheet
