DYKDDDDK-tag monoclonal antibody (clone M2.1), hIgG1

The in vivo grade recombinant anti-DYKDDDDK-Tag human IgG1 monoclonal antibody (clone M2.1) is a chimeric mouse/human monoclonal antibody produced in mammalian cells. It consists of mouse variable regions and human IgG1 kappa constant regions. 
Specificity/Sensitivity: detection and purification of DYKDDDDK fusion proteins no matter if the DYKDDDDK sequence is present at the N-terminus, Met N-terminus, C-terminus, or an internal site of the fusion proteins.
Applications: ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation, electron microscopy, supershift assays, protein purification if conjugated with agarose or magnetic beads, and isotype controls. 
Reactivity: Human, Mouse, Rat, Others.
Purification: The DYKDDDDK-tag antibody was affinity-purified from supernatant of mammalian cells cultured in a chemically defined medium by affinity-chromatography using protein A.
Form of Antibody: 1 mg/ml in 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Clone: M2.1.
Isotype: human IgG1 kappa.

Shipping: The in vivo grade recombinant anti-DYKDDDDK-Tag human IgG1 monoclonal antibody (clone M2.1) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.