Hamster IgG1 isotype control antibody

Both Armenian hamster IgG1 isotype control and Syrian hamster IgG1 isotype control (natural and engineered hamster IgG1 isotype controls) are useful for in vivo functional study using mouse and rat research model among a number of in vitro and in vivo research applications from ELISA, Western Blot (WB) to cell-based assays. The amino acid and DNA sequences of hamster IgG antibodies are different from the sequences of mouse and rat antibodies. Most of hamster antibodies are used to study mouse and rat target proteins. The ideal recombinant hamster IgG1 isotype control for in vivo use has low or no specific binding to any mouse and rat sample, and murinized or completely mouse variable regions in addition to the hamster IgG1 kappa constant regions.

There are more and more IgG antibody formats with Fc engineered. The formats with the Fc silenced, Fc silent, or Fcs with silent effector function is the most popular. Thus, we also provide Fc silent effector function hamster IgG isotype controls, including hamster IgG1 and IgG2 kappa isotype controls.

Please contact us to ask for a quote for the Fc silenced hamster IgG1 isotype control mutants, hamster IgG1 isotype control and Fc silent mutants with Avi-, His-, and Flag-tags, and biotinylated hamster IgG1 isotype control and mutants. A variety of conjugates (such as dyes and fluorophores) with the hamster IgG isotype control and Fc effector-silent mutants are available.

The in vivo grade recombinant Armenian hamster IgG1 isotype control antibody (hamster IgG isotype control) was produced in mammalian cells.
Immunogen: N/A.
Clone: 12B9.
Isotype: hamster IgG1, kappa.
Applications: an isotype-matched negative control for hamster IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The recombinant hamster IgG1 isotype control is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.

Background

Hamster IgG antibodies are classified to three subclasses mainly based on the isotype of the heavy chain (IgG1, IgG2, and IgG3 for the heavy chain; kappa and lamda for the light chain), of which kappa is the most abundantnamed in serum. IgG antibodies are large molecules of about 150 kDa composed of four peptide chains, two identical heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The four peptides are arranged in a Y-shape tetramer by disulfide bonds formed between the two heavy chains and between a heavy chain and a light chain.

Hamster IgG1 antibodies can be purified by the protein G beads, and relatively lower yield by the protein A beads.