Hamster IgG2 isotype control antibody

The ideal recombinant hamster IgG2 isotype control has low or no specific binding to any mouse and rat sample, and murinized or completely mouse variable regions in addition to the hamster IgG2 kappa constant regions. The amino acid and DNA sequences of mouse IgG antibodies are close to rat sequences but far away from much hamster ones. Most of hamster antibodies are used to study mouse and rat target proteins. Armenian hamster IgG2 isotype control and mutants (natural and engineered IgG2 isotype controls) are useful for functional study using mouse and rat model among a number of in vitro and in vivo research applications including ELISA, Western Blot (WB), and various cell-based assays.

Please contact us to ask for a quote for the engineered hamster IgG2 isotype control mutants, hamster IgG2 isotype control and Fc 'effector-silent' mutants with Avi-, His-, and Flag-tags, and biotinylated hamster IgG2 isotype control and silent Fc mutants. A variety of conjugates (such as dyes and fluorophores) with the hamster IgG2 isotype control and Fc silenced mutants are available.

In Vivo Grade Recombinant Armenian Hamster IgG2 Isotype Control Antibody

The in vivo grade recombinant Armenian hamster IgG2 isotype control antibody was produced in mammalian cells.
Immunogen: N/A.
Clone: 12B9.
Isotype: hamster IgG2, kappa.
Applications: an isotype-matched negative control for hamster IgG2 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The recombinant hamster IgG2 isotype control is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.

Background

Hamster IgG antibodies are classified to three subclasses mainly based on the isotype of the heavy chain (IgG1, IgG2, and IgG3 for the heavy chain; kappa and lamda for the light chain), of which kappa is the most abundantnamed in serum. IgG antibodies are large molecules of about 150 kDa composed of four peptide chains, two identical heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The four peptides are arranged in a Y-shape tetramer by disulfide bonds formed between the two heavy chains and between a heavy chain and a light chain.

Hamster IgG2 antibodies can be purified by the protein G beads, and relatively lower yield by the protein A beads.