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    Apenzy Biosciences

    Human FGF-7/KGF Protein (KGF-HE101)

    Human FGF-7/KGF Protein (KGF-HE101)

    $300.00
    $300.00
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    Size

    Product Description

    		 Recombinant Human FGF-7/KGF Protein is expressed from E.coli with His tag at the N-Terminus.It contains Cys32-Thr194.

    Product Background

    		 The expression patterns of mRNAs encoding Fibroblast Growth Factor-7 (FGF-7) and its high affinity receptor suggested that FGF-7 signaling may play a role in regulating ureteric bud growth.t Results of these studies demonstrate that the developing ureteric bud and mature collecting system of FGF-7-null kidneys is markedly smaller than wild type. FGF-7 levels modulate the extent of ureteric bud growth during development and the number of nephrons that eventually form in the kidney.

    Product Category

    		 Recombinant Protein

    Protein

    		 FGF-7/KGF

    Synonyms

    		 FGF-7; HBGF-7; KGF; FGF7

    Accession

    Species

    		 Human

    Biotinylated

    		 no

    Amino Acid Range

    		 Cys32-Thr194

    Molecular Weight

    		 The protein has a predicted MW of 20.1 kDa same as Bis-Tris PAGE result.

    Activity

    		 Immobilized Human FGF-7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human FGFR2 beta (IIIb), hFc Tag with the EC50 of 1.22ug/ml determined by ELISA (QC Test). The affinity constant of 18.91 nM as determined in SPR assay (Biacore T200). See testing image for detail.

    Product Tag

    		 N-His

    Expression System

    		 E.coli

    Purity

    		 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

    Endotoxin

    		 Less than 0.1EU per ug by the LAL method.
    Form
    		 Lyophilized
    Shipping
    		 Shipped at ambient temperature.
    Formulation
    		 Lyophilized from 0.22 um filtered solution in 50mM Tris, 250mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
    Reconstitution
    		 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
    Stability And Storage
    		 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    Human FGF-7, His Tag immobilized on CM5 Chip can bind Human FGFR2 beta (IIIb) , His Tag with an affinity constant of 18.91 nM as determined in SPR assay (Biacore T200). Human FGF-7, His Tag immobilized on CM5 Chip can bind Human FGFR2 beta (IIIb) , His Tag with an affinity constant of 18.91 nM as determined in SPR assay (Biacore T200).
    Immobilized Human FGF-7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human FGFR2 beta (IIIb), hFc Tag with the EC50 of 1.22ug/ml determined by ELISA (QC Test). Immobilized Human FGF-7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human FGFR2 beta (IIIb), hFc Tag with the EC50 of 1.22ug/ml determined by ELISA (QC Test).
    Human FGF-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human FGF-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
    The purity of Human FGF-7 is greater than 95% as determined by SEC-HPLC. The purity of Human FGF-7 is greater than 95% as determined by SEC-HPLC.
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