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    Apenzy Biosciences

    Human HGF Protein (HGF-HM101)

    Human HGF Protein (HGF-HM101)

    $255.00
    $255.00
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    Size

    Product Description

    		 Recombinant Human HGF Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln32-Ser728.

    Product Background

    		 Hepatocyte growth factor (HGF) is an important component of the pathophysiology of IR, with increased levels in most common IR conditions, including obesity. HGF has a role in the metabolic flux of glucose in different insulin sensitive cell types.

    Product Category

    		 Recombinant Protein

    Protein

    		 HGF

    Synonyms

    		 Hepatopoietin-A; SF; HPTA; HGF; DFNB39; F-TCF; F-TCFB; HGFB

    Accession

    Species

    		 Human

    Biotinylated

    		 no

    Amino Acid Range

    		 Gln32-Ser728

    Molecular Weight

    		 The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain.The protein has a predicted MW of 79.6 kDa (after cleavage of the signal peptide). Due to glycosylation, the protein migrates to 60-65 kDa and 30-40 kDa corresponding to α chain and β chain based on Bis-Tris PAGE result.

    Activity

    		 Immobilized Human HGF, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human HGF R, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail.

    Product Tag

    		 C-His

    Expression System

    		 HEK293

    Purity

    		 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

    Endotoxin

    		 Less than 0.1EU per ug by the LAL method.
    Form
    		 Lyophilized
    Shipping
    		 Shipped at ambient temperature.
    Formulation
    		 Lyophilized from 0.22um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
    Reconstitution
    		 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
    Stability And Storage
    		 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    Immobilized Human HGF, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human HGF R, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. Immobilized Human HGF, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human HGF R, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.
    Human HGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human HGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
    The purity of Human HGF Protein is greater than 95% as determined by SEC-HPLC. The purity of Human HGF Protein is greater than 95% as determined by SEC-HPLC.
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