Human IgG3 isotype control antibody

The recombinant human IgG3 isotype control (hIgG3 kappa isotype control antibody) and mutants are produced in mammalian cells (HEK293 and CHO cells). The recombinant human IgG3 isotype control has low or no specific binding to any human sample, and humanized or completely human variable regions in addition to the human IgG3 kappa constant regions. The human IgG3 kappa isotype control and mutants such as Fc-silenced can be used in ELISA, Western blot (WB), the cell-based assay or animal model as the antibody negative control.

The isotype controls are normally used to differentiate any non-specific background signal from the specific antibody signal, including the immunogenicity if used in animals. Most in vivo functional grade human IgG antibodies are used in mice for in vivo studies. Besides the antibody Complementarity-Determining Regions (CDRs), two human or humanized variable regions including the one in the human or humanized IgG isotype control do not necessarily induce the same immune response in mice because in some cases, even one amino acid difference can make difference. Mouse variable regions can be used to show the potential immunogenicity induced by the human variable regions in the human or humanized IgG antibodies to test. Thus, in some cases, it is better to use the mouse variable region in the human IgG isotype controls. We also produce and sell recombinant human IgG isotype controls from the popuplar mouse hybridoma clones, MOPC21 and C1.18.4, as well as recombinant human IgG1 kappa isotype controls against KLH, TNP, DNP,  GST, HEL, His-tag, Flag, c-myc, HA, GFP, and unknown specificity.

Please contact us to ask for a quote for the Fc silenced human IgG3 isotype control mutants, hIgG3 isotype control and silent Fc mutants with Avi-, His-, and Flag-tags, and biotinylated hIgG3 isotype control and Fc silent mutants. A variety of conjugates (such as a fluorophore) with the human IgG3 isotype control and Fc effector-silent mutants are available.

 In Vivo Grade Recombinant Human IgG3 kappa Isotype Control Antibody (hIgG3 Isotype Control)

The in vivo grade recombinant human IgG3 kappa isotype control antibody (hIgG1 isotype control) was produced in mammalian cells.
Immunogen: N/A.
Clone: 4F17.
Isotype: human IgG1, kappa.
Applications: an isotype-matched negative control for human IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.

Background

Isotype control antibodies (negative control antibodies or reference antibodies) are frequently used in various antibody-related in vivo and in vitro experiments such as ELISA, Western blot, immunofluorescence, immunohistochemistry, immunoprecipitation, and flow cytometry. Isotype control antibodies are used as negative controls of primary antibodies to check presence of non-specific binding to Fc receptors on the cell surface, other cellular proteins, carbohydrates, and lipids. Thus, the isotype control antibody normally matches the host species and isotype, including light chains (such as human IgG1 kappa antibody), concentration, and conjugation format of the experimental primary antibody.