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Apenzy Biosciences
Human IL-7 Protein
Human IL-7 Protein
Regular price
$988.00
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$988.00
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Overview
| Product Description | Recombinant Human IL-7 Protein is expressed from E.coli without tag.It contains Asp26-His177. |
Product Background |
Interleukin-7 (IL7) plays a nonredundant role in T cell survival and homeostasis, which is illustrated in the severe T cell lymphopenia of IL7-deficient mice, or demonstrated in animals or humans that lack expression of either the IL7Rα or γ c chain, the two subunits that constitute the functional IL7 receptor. |
Product Category |
Recombinant Protein |
Specifications
Target |
IL-7 |
| Synonyms | IL7; Interleukin-7; IL-7; Lymphopoietin-1; PBGF |
Gene/Protein ID |
P13232 |
Species |
Human |
Exact Sequence |
Asp26-His177 |
| Molecular Weight | The protein has a predicted MW of 17.5 kDa same as Tris-Bis PAGE result. |
Activity |
Immobilized Human IL-7 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 52.5ng/ml determined by ELISA. The affinity constant of 4.18 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
No Tag |
Express System |
E.coli |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in 25mM PB, 250mM NaCl (pH 6.8). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 25mM PB, 250mM NaCl (pH 6.8). |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |