Human OPG-Fc protein

Introduction

Osteoprotegerin (OPG) also known as Osteoclast Inhibiting Factor (OCIF) or Osteoclast Binding Factor (OBF), is a key factor inhibiting the differentiation and activation of osteoclasts, and is therefore essential for bone resorption. Osteoprotegerin is a glycoprotein belonging to the TNF receptor family. As a so called soluble "decoy" receptor, Osteoprotegerin inhibits the binding of RANK to RANKL and thus inhibits the recruitment, proliferation and activation of osteoclasts.

Recombinant Human OPG-Fc Fusion Protein

Source: HEK 293-derived.
The Osteoprotegerin / OPG / TNFRSF11B / OCIF / OBF activity is calculated by its ability to inhibit TRAIL-mediated cytotoxicity using L929 mouse fibrosarcoma cells treated with TRAIL. The ED50 for this effect is typically 5 ng/ml.
Endotoxin: < 5.0 EU per 1 ug of the protein by the LAL method.
Purity: > 90% by SDS-PAGE and RP-HPLC.
Formulation: In 1% Surcose, 100 mM NaCl, 25 mM L-arginine hydrochloride, and 25 mM sodium phosphate. pH 6.3.

Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.