Recombinant Human PD1-His fusion protein

Background

Programmed death-1 (PD-1) is a type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses. Members of the CD28/CTLA-4 family both up-regulate (CD28 and ICOS) and downregulate T cell activation (CTLA-4 and PD-1). PD-1 is expressed on activated T cells, B cells, myeloid cells and on a subset of thymocytes. Mouse and human PD-1 share approximately 60% amino acid sequence identity.

Recombinant Human PD1-His fusion protein

Source: HEK 293 cell-derived.

The recombinant human PD1-His fusion protein is the human PD1 ECD (amino acids 25-167) fused with a polyhistidine tag at the C-terminus.
Purity: >95% by SDS-PAGE under reducing conditions.
Activity: The recombinant human PD1-his fusion protein binds the recombinant human PDL1 protein at sub-micromolar range.
Formulation: 0.2 μM filtered solution of 30 mM HEPES, 150 mM NaCl, pH 7.
Endotoxin Level: Less than 1 EU/ug of protein as determined by LAL method.

Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.