In Vivo Grade Recombinant Rat IgG2b Isotype Control Antibody, Endotoxin 0.05 EU/mg

Rat IgG2b isotype control and mutants (natural and engineered rtIgG2b isotype controls) are useful for functional study using animal model among a number of in vitro and in vivo research applications. Rat IgG2b antibodies are more like mouse IgG2a and IgG2b, and human IgG1 antibodies, which are mostly used by the therapeutic antibody drugs. Most of rat antibodies are used to study mouse target proteins.

There are more and more IgG antibody formats with Fc engineered. The format with the Fc silenced, Fc silent, or Fcs with silenced effector function is the most popular. We provide Fc effector-silent rat IgG2b isotype controls.

Please contact us to ask for a quote for the engineered rat IgG2b isotype control mutants, rtIgG2b isotype control and mutants with Avi-, His-, and Flag-tags, and biotinylated rtIgG2b isotype control and mutants. A variety of conjugates (such as dyes and fluorophores) with the rat IgG2b isotype control and Fc mutants are available.

: In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody (rtIgG2b Isotype Control)

The in vivo grade recombinant rat IgG2b isotype control antibody (rtIgG2b isotype control) was produced in mammalian cells.
Immunogen: N/A.
Clone: 12B9.
Isotype: rat IgG2b, kappa.
Applications: an isotype-matched negative control for rat IgG2b kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The recombinant rtIgG2b isotype control is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.

Background

Rat IgG antibodies are classified to four subclasses mainly based on the isotype of the heavy chain (IgG1, IgG2a, IgG2b, and IgG2c for the heavy chain; kappa and lamda for the light chain), of which IgG1 and kappa are the most abundantnamed in serum. IgG antibodies are large molecules of about 150 kDa composed of four peptide chains, two identical heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The four peptides are arranged in a Y-shape tetramer by disulfide bonds formed between the two heavy chains and between a heavy chain and a light chain.