Mouse CD8 T cell targeted LNP kit (SV105Delivery)

The ready-to-use LNP kit enables specific delivery to mouse CD8 T cells. It is intended for research use only, supporting proof-of-concept studies and screening of mRNA constructs ex vivo (in mouse splenic cells) and in vivo (in mice or humanized mice).

Kit Components

SV105-36 Delivery for mouse CD8 T Cells delivery of 36 μg mRNA. The kit contains 3 x 300μL Lipid Mixture, 1 x 1.5mL Formulation Buffer, 1 x 3mg Reagent A, 1 x 400 μL Reagent B-5. For Research Use Only.

SV105-120 Delivery Plus for mouse CD8 T Cells delivery of 120 μg mRNA. The kit contains 5 x 600μL Lipid Mixture, 2 x 2mL Formulation Buffer, 2 x 3mg Reagent A, 2 x 600μL Reagent B-5. For Research Use Only

Cell-type specificity: Enabling targeted delivery to mouse CD8 T cells with exceptional selectivity.

Mouse CD8 T cell targeted SV105Delivery^™ Kits information andSupporting Data

Table 3 The Characterizations of mouse CD8 T cell targeted LNP-mRNA generated by SV105 Delivery^MT Kits

The kits exhibit excellent encapsulation efficiency, generate nanoparticles with a uniform average size of approximately 100 nm, and maintain a low polydispersity index (PDI).

Figure 5. Stability of mouse CD8 T cell targeted LNP-mRNA generated by SV105Delivery^™ Kits. The mouse CD8 T cell targeted LNP-mRNA underwent three freeze–thaw cycles prior to transfect mouse splenic cells. Shown are nanoparticle size (A), polydispersity index (PDI) (B), and transfection efficiency in mouse splenic cells (C).

Figure 6. The SV105Delivery^™ kit demonstrated robust transfection efficiency and cell-type specificity in mouse splenic cells. In vitro transfection efficacy: Mouse splenic cells were transfected with LNP–mRNA encoding eGFP, formulated using the SV105Delivery^™ kit as indicated. Shown are representative FACS plots (A) and the percentage of GFP⁺ cells (B) one day post-transfection, as determined by flow cytometry.

Store Temperature

Formulation Mixture: -80°C

Formulation Buffer: Room Temperature

Reagent A: Room Temperature

Reagent B-5: 4°C