SunVax mRNA Therapeutics
Mouse T cell targeted LNP kit (SV101Delivery)
Mouse T cell targeted LNP kit (SV101Delivery)
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The ready-to-use LNP kit enables specific delivery to mouse T cells both in vivo and in vitro. It is intended for research use only, supporting proof-of-concept studies and screening of mRNA constructs in vitro (in mouse splenic cells or human PBMCs) and in vivo (in mouse or humanized mouse).
Kit Components
SV101Delivery: For Mouse T Cells delivery of 36 μg mRNA. The kit contains 3 x 300μL Lipid Mixture, 1 x 1.5mL Formulation Buffer, 1 x 3mg Reagent A, 1 x 400 μL Reagent B-1. For Research Use Only
SV101Delivery Plus: For Mouse T Cells delivery of 120 μg mRNA. The kit contains 5 x 600μL Lipid Mixture, 2 x 2mL Formulation Buffer, 2 x 3mg Reagent A, 2 x 600μL Reagent B-1. For Research Use Only.
Cell-type specificity: Enabling targeted delivery to mouse T cells with exceptional selectivity.
Mouse T cell targeted SV101Delivery Kits information and Supporting Data
Table 1 The Characterizations of mouse T cell targeted LNP-mRNA generated by SV101Delivery™ Kits
|
LNP-mRNA |
EE (%) |
Size (nm) |
PDI |
|
SV101 Delivery™ |
99.2±0.6 |
99.2±0.9 |
0.17±0.01 |
The kits exhibit excellent encapsulation efficiency, generate nanoparticles with a uniform average size of approximately 100 nm, and maintain a low polydispersity index (PDI).
Figure 1. Stability of mouse T cell targeted LNP-mRNA generated by SV101Delivery™ Kits. The mouse T cell targeted LNP-mRNA underwent three freeze–thaw cycles prior to transfect mouse splenic cells. Shown are nanoparticle size (A), polydispersity index (PDI) (B), and transfection efficiency in mouse splenic cells (C).
Figure 2. The SV101Delivery™ kit demonstrated robust transfection efficiency and cell-type specificity in mouse splenic cells. In vitro transfection efficacy: Mouse splenic cells were transfected with LNP–mRNA encoding eGFP, formulated using the SV101Delivery™ kit as indicated. Shown are representative FACS plots (A) and the percentage of GFP⁺ cells (B) one day post-transfection, as determined by flow cytometry.
Store Temperature
Formulation Mixture: -80°C
Formulation Buffer: Room Temperature
Reagent A: Room Temperature
Reagent B-1: 4°C