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    Yeasen

    Polyethylenimine Linear(PEI) MW40000 (rapid lysis)

    Polyethylenimine Linear(PEI) MW40000 (rapid lysis)

    $162.00
    $162.00
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    Description

    PEI 40000 is a highly charged cationic polymer with a molecular weight of 40,000 that binds negatively charged nucleic acid molecules very easily, forming a complex and allowing the complex to enter cells. PEI 40000 is a transient transfection reagent with low cytotoxicity, high transfection efficiency, and high gene expression efficiency in cells such as HEK293 and CHO.

    PEI 40000 has many advantages over PEI 25000 Transfection Reagent as below:

    1. PEI 40000 is easy to dissolve and can be directly dissolved in water. PEI 25000 needs to first adjust the water to weak acid to help it dissolve, and then use NaOH to adjust the pH to neutral.

    2. PEI 40000 is easy to operate, easier to use, and has a better transfection effect than PEI 25000; 3. PEI 25000 contains 4-11% propionyl residues, which can prevent the polymer backbone from binding to DNA. Compared to PEI 25000, PEI 40000 is a complete shed construction, so its performance is consistently efficient.

    This product is an instant type, which dissolves quickly and is easy to prepare.

    Feature

    • Exceptional Efficiency: Superior transfection performance in the broad range of cell lines, including transient transfection and stable transfection
    • Wide Adaptability: Excellent transfection efficiency in a variety of cell lines and high levels of recombinant protein expression
    • Low Toxicity: The activities of the diverse cells almost unaffected by transfection reagents from YEASEN
    • Simple Operation: Proven efficacy in the presence of serum—eliminates the need to change media following transfection
    • Cost Effective: Competitive transfection effect with more affordable prices

    Application

    • Cell Transfection

    Specification

    Name Polyethylenimine Linear (PEI) 40000 
    CAS No. 49533-93-7
    Molecular formula (CH2CH2NH)n
    Molecular weight 40,000
    Solubility Soluble in water, insoluble in organic solvents: benzene, ether and acetone
    Structure
    Form White or off-white solid
    Serum Compatible Yes
    Cell Type Established Cell Lines
    Sample Type Plasmid DNA
    Transfection Technique PEI-Based Transfection

    Components

    Components No. Name 40816ES02 40816ES03 40816ES08
    40816 Polyethylenimine Linear (PEI) MW40000(rapid lysis) 100 mg 1 g 5×1 g

    Shipping and Storage

    The product is shipped at room temperature and the powder can be stored at 2-8ºC for two years. The stock solution is stored at 2-8 ºC for 3 months.

    Figures

    • Demonstration of Transfection Effect

    Figure 1. The transfection efficiency of Polyethylenimine Linear (PEI) MW40000 Reagent was shown above.

    Polyethylenimine Linear (PEI) MW40000 Reagents were used to transfect the HEK293 cell line and CHO-K1 cell line in a 96-well format. GFP expression was analyzed 48 hours posttransfection.

    • Validated Cell Lines
    Product Name Polyethylenimine Linear (PEI) MW40000 (Rapid Lysis)
    Validated Cell Lines COS-1 CHO-K1 HEK293 HepG2
    COS-7 Hela HEK293T NIH-3T3

     

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