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    Yeasen

    Hieff Trans PEI Transfection Reagent

    Hieff Trans PEI Transfection Reagent

    $225.00
    $225.00
    Sale Sold out
    Size

    Description

    This product is a Linear Polyethylenimine (PEI) after optimization and transformation, with a concentration of 1 mg/mL. This product is purely chemically synthesized and does not contain animal-derived ingredients. It is particularly suitable for co-transfection of multiple plasmids, for the production of recombinant virus vectors and the instantaneous expression of recombinant proteins. This product has low cytotoxicity, high transfection efficiency, and is compatible with antibiotics. It has high gene expression efficiency in HEK293 and other cells.

    Feature

    • Small scale and large-scale production of various viruse vectors and transient expression of proteins suitable for adherent and suspension systems
    • High transfection efficiency, less plasmid usage and high-cost performance
    • Compatible with a variety of commercial medium
    • Sufficient capacity and stable batch

    Application

    • Cell Transfection

    Specification

    Physical and chemical Properties Information
    Form Liquid
    Serum Compatible Yes
    Cell Type Established Cell Lines
    Sample Type Plasmid DNA
    Transfection Technique PEI-Based Transfection

    Components

    Components No. Name 40820ES04 40820ES10 40820ES60
    40820 Hieff Trans PEI Transfection Reagent 1.5 mL 10 mL 100 mL

    Storage

    The product could be stored at 2-8ºC for two years. Do not freeze!

    Figures

    • Quality
    Item

    Specifications

    R&D

    GMP

    Clarity

    Colorless liquid

    √

    √

    Activity

    Transfection efficiency>70%

    Cell viability>80%

    √

    √

    Endotoxin

    <0.5 EU/mL

    √

    √

    Sterility

    Sterile after 14 days

    √

    √

    Mycoplasma

    Absence

    √

    √

    pH

    <7.0

     

    √

    Osmolality

    ≤30 mOsm/kg

     

    √

    Impurity distribution

    No residual acetonitrile, methanol, ether, toluene

     

    √

     

    • Transfection Efficiency

    Figure 1. The transfection efficiency of Hieff Trans PEI Transfection Reagent-GMP.

    Hieff Trans PEI Transfection Reagent-GMP was used to transfect the suspension cell HEK293F cell line and adherent cell HEK293T cell line in a 6-well format. AAV-GFP expression was analyzed 48 hours post-transfection. (the ratio of the plasmid to reagent is 1:1)

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