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    Yeasen

    Proteinase K

    Proteinase K

    $95.00
    $95.00
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    Description

    Proteinase K is a serine protease with wide cleavage activity, which can cleave the carboxyl-terminal peptide bond of aliphatic and aromatic amino acids. Its relative molecular weight is about 29.3 kDa. Proteinase K is widely used in the preparation of chromosomal DNA for pulse electrophoresis, Western blotting, and the removal of a nuclease from DNA and RNA preparation. Denaturant such as SDS (1%) can improve its activity. In addition, the common working concentration is 50-100 μg/mL, and the specific working concentration is according to whether the buffer contains SDS, urea, pH, temperature, and other factors.

    Applications

    • Genomic DNA extraction
    • Digestion and removal of the enzyme

    Specifications

    Source Yeast
    Specific Activity ≥30 U/mg
    Unit Definition One unit is defined as the amount of proteinase K that will liberate 1μmoL tyrosine per minute at 37℃ @ pH 7.5.
    Purification Ni column affinity purification
    Reaction Condition The reaction buffer should be incubated at 37℃ -70℃ for 1h, and the recommended reaction temperature is 70℃.
    Inactivation method The reaction system can be inactivated by adding PMSF or DFP inhibitor and partially inactivated by incubation at 65℃ for 10-15 min.
    Activity Range It has activity in a wide range of pH (4.0-12.0) and the optimal pH range is 7.5-9.0.
    RNase and DNase None

    Components

    Components No. Name 10401ES60 10401ES80 10401ES90 10401ES98
    10401 Proteinase K 100 mg 1 g 5 g 100 g

    Shipping and Storage

    The product is shipped with ice packs and can be stored at 2℃ ~ 8℃ for three years.

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