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    Hieff UNICON Universal Blue qPCR SYBR Master Mix

    Hieff UNICON Universal Blue qPCR SYBR Master Mix

    $90.00
    $90.00
    Sale Sold out
    Size

    Description

    Hieff Unicon Universal Blue qPCR Master Mix (Dye Based) is a pre-solution for 2×real-time quantitative PCR amplification characterized by high sensitivity and specificity, is blue in color, and has the effect of sample addition tracing. The core component Hieff Unicon™ Taq DNA polymerase uses antibody hot start to effectively inhibit non-specific amplification due to primer annealing during sample preparation. At the same time, the formula adds the promoting factors to improve the amplification efficiency of PCR reaction and equalize the amplification of genes with different GC contents (30 ~ 70%), so that quantitative PCR can obtain a good linear relationship in a wide quantitative region. This product contains special ROX Passive Reference Dye, which is applicable to most qPCR instruments. It is not necessary to adjust the concentration of ROX on different instruments. It is only necessary to add primers and templates to prepare the reaction system for amplification.

    Features

    • Built-in visual indicator: It contains an inert blue dye that does not interfere with real-time PCR and provides higher visibility in tubes or Wells.
    • High specificity: It contains Taq DNA polymerase and is tightly controlled by an antibody-mediated heat-priming mechanism, thereby avoiding nonspecific amplification.
    • Wide instrument compatibility: It is compatible with most qPCR instruments and eliminates the need to adjust the concentration of ROX on different instruments

    Applications

    • Gene Expression

    Specifications

    Concentration 2×
    Detection method SYBR
    PCR method qPCR
    Polymerase Taq DNA polymerase
    Type of sample DNA
    Application equipment Most qPCR instruments
    Product Type SYBR premix for real-time fluorescence quantitative PCR
    Apply to (application) Gene Expression

    Components

    Components No. Name 11184ES03 11184ES08 11184ES50 11184ES60
    11184 Hieff Unicon™ Universal Blue qPCR Master Mix (Dye Based) 1 mL 5×1 mL 50×1 mL 100×1 mL

    Shipping and Storage

    The product is shipped with ice packs and can be stored at -15℃ ~ -25℃ for 18 months. The product contains fluorescent dyes, so it is necessary to avoid strong light irradiation when storing or preparing the reaction system.

    Figures 

    Figure 1. High sensitivity: the ability to detect a single copy.

     

    Figure 2. High resolution & Excellent reproducibility of duplicate wells.

     

     Figure 3. Excellent detection rate and specificity: broadly suited for the amplification of 30-70% GC.

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