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    Yeasen

    T7 RNA Polymerase GMP-grade (50 U/μL)

    T7 RNA Polymerase GMP-grade (50 U/μL)

    $55.00
    $55.00
    Sale Sold out
    Size

    Description

    This product is a bacteriophage T7 RNA polymerase derived from recombinant protein expression in Escherichia coli. It catalyzes the 5'→3' synthesis of RNA on double-stranded DNA from its T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') and uses NTPs as substrates. The DNA with double-stranded linear blunt ends or 5'protruding ends can be used as templates for T7 RNA polymerase, so linearized plasmids and PCR products can be used as templates for in vitro synthesis of RNA.

    Note: G* is the first base of the RNA transcript.

    This product is produced in accordance with GMP regulations and provided in liquid form.

    Feature

    • Synthesize long transcripts and short transcripts, RNA can be produced 100-200 μg with 1 μg of DNA template
    • Higher yield and easy to operate
    • Lower endotoxins
    • Tested for the absence of endonucleases, exonucleases, RNases

    Application

    • Radiolabeled RNA probe preparation
    • RNA generation for studies of RNA structure, processing and catalysis
    • Expression control via anti-sense RNA
    • mRNA, sgRNA synthesis

    Specification

    Source Recombinant E. coli with T7 RNA Polymerase gene
    Optimum Temperature 37℃
    Storage Buffer 50 mM Tris-HCl, 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0.1% Triton X-100，50% (v/v) glycerin，pH7.9 at 25℃
    Unit Definition The amount of enzyme required to incorporate 1 nmol of [3H] GMP into the acid-insoluble precipitate within 1 hour at 37°C and pH 8.0 is defined as 1 unit.

    Components

    Components No. Name 10624ES90 (5,000 U) 10624ES97 (50,000 U)
    10624 T7 RNA Polymerase GMP-grade (50 U/μL) 100 μL 1 mL

    Shipping and Storage

    T7 RNA Polymerase GMP-grade products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.

     

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